Effects induced by Apis mellifera venom and its components in experimental models of nociceptive and inflammatory pain

AbstractThe effects induced by Apis mellifera venom (AMV), melittin-free AMV, fraction with molecular mass < 10 kDa (F<10) or melittin in nociceptive and inflammatory pain models in mice were investigated. Subcutaneous administration of AMV (2, 4 or 6 mg/kg) or melittin-free AMV (1, 2 or 4 mg/kg) into the dorsum of mice inhibited both phases of formaldehyde-induced nociception. However, F<10 (2, 4 or 6 mg/kg) or melittin (2 or 3 mg/kg) inhibited only the second phase. AMV (4 or 6 mg/kg), but not F<10, melittin-free AMV or melittin, induced antinociception in the hot-plate model. Paw injection of AMV (0.05 or 0.10 mg), F<10 (0.05 or 0.1 mg) or melittin (0.025 or 0.050 mg) induced a nociceptive response. In spite of inducing nociception after paw injection, scorpion (Tityus serrulatus) or snake (Bothrops jararaca) venom injected into the dorsum of mice did not inhibit formaldehyde-induced nociception. In addition, AMV (6 mg/kg), but not F<10 (6 mg/kg) or melittin (3 mg/kg), inhibited formaldehyde paw oedema. Concluding, AMV, F<10 and melittin induce two contrasting effects: nociception and antinociception. AMV antinociception involves the action of different components and does not result from non-specific activation of endogenous antinociceptive mechanisms activated by exposure to noxious stimuli

Morfologia dos vasos da base do encéfalo do quati (Nasua nasua)

Estudou-se a morfologia do encéfalo de Nasua nasua - quati, buscando comparar estes achados com outras espécies já descritos. Foram utilizados cinco encéfalos de quatis, provenientes do Criatório Científico (Cecrimpas), Unifeob. Os animais foram eutanásiados de acordo com a legislação (Cobea). Canulou-se a artéria carótida comum e a veia jugular externa sentido cranial, injetou-se solução de látex/bário corado de vermelho na artéria carótida, e solução de látex corado de azul na veia jugular. Em seguida os animais fixados em solução de formaldeído a 10%. O encéfalo tem sua nutrição dependente de quatro artérias, as artérias carótidas internas e as artérias vertebrais direitas e esquerdas. Esses vasos compuseram o circuito basilar e carotídeo que se anastomosam através das artérias cerebrais caudais. Correm na base do encéfalo sob a meninge pia mater

The Brazilian Soil Spectral Library (BSSL): A general view, application and challenges

Made available in DSpace on 2019-10-06T16:42:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-11-15Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The present study was developed in a joint partnership with the Brazilian pedometrics community to standardize and evaluate spectra within the 350–2500 nm range of Brazilian soils. The Brazilian Soil Spectral Library (BSSL) began in 1995, creating a protocol to gather soil samples from different locations in Brazil. The BSSL reached 39,284 soil samples from 65 contributors representing 41 institutions from all 26 states. Through the BSSL spectra database, it was possible to estimate important soil attributes, such as clay, sand, soil organic carbon, cation exchange capacity, pH and base saturation, resulting in differences among the multi-scale models taking Brazil (overall), regional and state scale. In general, spectral descriptive and quantitative behavior indicated important relationship with physical, chemical and mineralogical properties. Statistical analyses showed that six basic patterns of spectral signatures represent the Brazilian soils types and that environmental conditions explain the differences in spectra. This study demonstrates that spectroscopy analyses along with the establishment of soil spectral libraries are a powerful technique for providing information on a national and regional levels. We also developed an interactive online platform showing soil sample locations and their contributors. As soil spectroscopy is considered a fast, simple, accurate and nondestructive analytical procedure, its application may be integrated with wet analysis as an alternative to support the sustainable management of soils.Department of Soil Science Luiz de Queiroz College of Agriculture (ESALQ) University of São Paulo (USP), Ave. Pádua Dias 11, Cx. Postal 9Department of Soil Federal University of Santa Maria, Av. Roraima 1000Geographical Sciences Department Federal University of Pernambuco, Av. Ac. Hélio Ramos, s/nDepartment of Agronomy State University of Maringá, Av. Colombo 5790Department of Agriculture Biodiversity and Forestry Federal University of Santa Catarina, Rodovia Ulysses Gaboardi 3000 - Km 3Federal Rural University of Amazon, Ave. Presidente Tancredo Neves 2501Faculty of Agronomy and Veterinary Medicine University of BrasíliaEMBRAPA - Solos, R. Antônio Falcão, 402, Boa ViagemCenter of Nuclear Energy in Agriculture (CENA) USP, Av. Centenário 303CDRS/Secretary of Agriculture of São Paulo State, R. Campos Salles 507Department of Soils Federal University of Viçosa, Ave. Peter Henry Rolfs s/nEMBRAPA – Informática Agropecuária, Ave. André Tosello, 209Department of Nuclear Energy Federal University of Pernambuco, Av. Prof. Luis Freire 1000Department of Geography Federal University of Rio Grande do Norte, R. Joaquim Gregório s/nAgronomic Institute of Campinas (IAC), Ave. Barão de Itapura 1481Institute of Agricultural Sciences Federal Rural University of Amazônia, Ave. Presidente Tancredo Neves 2501, 66.077-830Department of Soil Science Federal University of LavrasFederal University of Mato Grosso, Cuiabá, Av. Fernando Corrêa da Costa 2367Department of Soils Federal Rural University of Rio de Janeiro, Rodovia BR 465, Km 07 s/nSoil and Water Sciences Department University of Florida, 2181 McCarty Hallr, PO Box 110290EMBRAPA - Solos, R. Jardim Botânico, 1024Department of Soils and Fertilizers School of Agricultural and Veterinary Studies São Paulo State University (FCAV-UNESP), Via de Acesso Prof. Paulo Donato Castellane s/nFederal University of Sergipe, Av. Marechal Rondon s/nGraduate Program in Earth Sciences (Geochemistry) Department of Geochemistry Federal Fluminense University, Outeiro São João Batista, s/nFederal Institute of the Southeast of Minas Gerais, R. Monsenhor José Augusto 204Federal University of Rio Grande do Norte, R. Joaquim Gregório s/nFederal University of PiauíEMBRAPA Milho e Sorgo, Rod MG 424 Km 45Institute of Agricultural Sciences Federal University of Jequitinhonha e Mucuri Valleys, Ave. Ver. João Narciso 1380Department of Biosystems Engineering ESALQ USP, Ave. Pádua Dias 11, Cx. Postal 9Federal University of Acre, Rodovia BR 364 Km 04Federal University of Amazonas, Av. General Rodrigo O. J. Ramos 1200EMBRAPA Clima Temperado, BR-392, km 78Department of Agronomy Federal Rural University of Pernambuco, R. Manuel de Medeiros s/nEMBRAPA Cocais, Quadra 11, Av. São Luís Rei de França 4Paraense Emílio Goeldi Museum, Av. Gov. Magalhães Barata 376Exata Laboratory, Rua Silvestre Carvalho Q 11Federal University of Rondônia, BR 364, Km 9.5Nacional Institute for Amazonian Research, Ave. André Araújo 2936Department of Forestry Sciences ESALQ-USP, Ave. Pádua Dias 11, Cx. Postal 9Department of Soils and Fertilizers School of Agricultural and Veterinary Studies São Paulo State University (FCAV-UNESP), Via de Acesso Prof. Paulo Donato Castellane s/nFAPESP: 2014/22262-0FAPESP: 2016/26176-6FAPESP: 2017/03207-

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora